Regulatory

Part:BBa_K817050:Design

Designed by: Anjui Wu   Group: iGEM12_NTU-Taida   (2012-09-18)

Novel thermal promoter, Phs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the paper, the clone the promoter upstream from transcription starting site till -85 region; however, we only clone up till -45 regions, in which -45~-35 region is proven to be critical to the promoter function.

For our functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/Thermal-Promoter wiki page].

Source

"Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase", Cell press, 1984 ATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGAT(+1)

References